4MO5
Crystal structure of AnmK bound to AMPPCP and anhMurNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-23 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.299, 70.407, 90.968 |
| Unit cell angles | 106.43, 104.43, 98.25 |
Refinement procedure
| Resolution | 44.806 - 1.750 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.20960 |
| Structure solution method | RIGID BODY REFINEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.059 | 0.493 |
| Number of reflections | 130828 | |
| <I/σ(I)> | 10 | 2.2 |
| Completeness [%] | 97.5 | 96.2 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 298 | 24% PEG4000, 0.2 M magnesium chloride, 0.1 M Tris, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
