4MO3

Crystal Structure of Porcine C2 Domain of Blood Coagulation Factor VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	2013-05-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	49.070, 68.940, 105.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.912 - 1.702
R-factor	0.1786
Rwork	0.175
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d7p
RMSD bond length	0.006
RMSD bond angle	1.218
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.912
High resolution limit [Å]	1.700	1.700
Rmerge	0.060
Number of reflections	19595
<I/σ(I)>	19.8
Completeness [%]	97.4
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.4	277	2:1 crystallization buffer (0.1 M magnesium acetate, 10% ethanol) to protein, crystals formed overnight with 0.1 M CHES buffer and 220 uL Hampton Research Al's oil over 400 uL reservoir, pH 10.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K