4MO0
Crystal structure of aIF1 from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98011 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 51.440, 51.440, 146.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.866 - 2.100 |
R-factor | 0.2203 |
Rwork | 0.220 |
R-free | 0.22790 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.073 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.900 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.063 | 0.595 |
Number of reflections | 7272 | |
<I/σ(I)> | 5.5 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 18.7 | 17.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 25% PEG3350, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |