4MNU

Crystal Structure of Uncharacterized SlyA-like Transcription Regulator from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	46.155, 84.362, 94.856
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.542 - 1.898
R-factor	0.181
Rwork	0.179
R-free	0.22200
Structure solution method	SAD and Molecular replacement
RMSD bond length	0.007
RMSD bond angle	1.064
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: dev_1367))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	29672
<I/σ(I)>	15.7	1.9
Completeness [%]	98.7	87.1
Redundancy	5.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2 M ammonium sulfate, 0.1 M sodium cacodylate pH 6.5, 30 %(w/v) PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K