4MNO

Crystal structure of aIF1A from Pyrococcus abyssi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	36.430, 46.770, 57.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.840 - 1.350
R-factor	0.1584
Rwork	0.157
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oqk
RMSD bond length	0.006
RMSD bond angle	1.025
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.930	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.054
Number of reflections	22282
Completeness [%]	99.8	99.8
Redundancy	4.7	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	278	20% PEG3350, 0.2 M formate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 278K