4MNN
The crystal structure of Sso1120 from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.100 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.909, 79.725, 67.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
Rwork | 0.193 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ayt |
RMSD bond length | 0.014 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.037 | 0.188 |
Number of reflections | 17444 | |
<I/σ(I)> | 35 | 4.5 |
Completeness [%] | 98.7 | 90 |
Redundancy | 5.4 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 13 % PEG 20000, 0.1 M MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |