4MND

Crystal structure of Archaeoglobus fulgidus IPCT-DIPPS bifunctional membrane protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 21 21 2
Unit cell lengths	41.369, 107.584, 123.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.850 - 2.660
R-factor	0.24561
Rwork	0.243
R-free	0.30006
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xme
RMSD bond length	0.013
RMSD bond angle	0.596
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	61.820
High resolution limit [Å]	2.650
Number of reflections	15952
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	295	PEG, sodium malonate, pH 7, in meso crystallization, temperature 22K, temperature 295K