4MN4

Structural Basis for the MukB-topoisomerase IV Interaction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	49.767, 103.737, 186.611
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.974 - 2.300
R-factor	0.2063
Rwork	0.204
R-free	0.24610
Structure solution method	SAD, MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3IBP AND IZVU
RMSD bond length	0.004
RMSD bond angle	0.613
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.974	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.062	0.395
Number of reflections	44001
<I/σ(I)>	17
Completeness [%]	98.1	88.5
Redundancy	3.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	291	212.5 mM lithium citrate tribasic tetrahydrate, 20% PEG3350, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.9	291	212.5 mM lithium citrate tribasic tetrahydrate, 20% PEG3350, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K