4MM2
Crystal structure of yeast primase catalytic subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.2826 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.370, 196.040, 53.960 |
Unit cell angles | 90.00, 114.72, 90.00 |
Refinement procedure
Resolution | 43.880 - 1.600 |
R-factor | 0.25689 |
Rwork | 0.255 |
R-free | 0.29293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mhq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.545 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.108 | 1.988 |
Number of reflections | 111075 | |
<I/σ(I)> | 10.17 | 0.89 |
Completeness [%] | 92.6 | 59.8 |
Redundancy | 5.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 298 | 0.1M Na-Citrate, 0.2M NDSB, 5mM CdCl2, 30% PEG 600, 10mM ZnCl2 , pH 5.5, VAPOR DIFFUSION, temperature 298K |