4MLO
1.65A resolution structure of ToxT from Vibrio cholerae (P21 Form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.337, 39.410, 80.238 |
Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
Resolution | 39.735 - 1.650 |
R-factor | 0.1692 |
Rwork | 0.168 |
R-free | 0.19440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbg |
RMSD bond length | 0.009 |
RMSD bond angle | 0.947 |
Data reduction software | XDS |
Data scaling software | SCALA (0.1.29) |
Phasing software | PHASER (2.5.5) |
Refinement software | PHENIX (dev_1444) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.470 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.081 | 0.680 |
Number of reflections | 35493 | |
<I/σ(I)> | 10.3 | 1.9 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 5% (w/V) PEG 5000, 10% (v/v) 2-propanol, 0.1 M MES, 0.2M magnesium chloride, pH 6.5, VAPOR DIFFUSION, temperature 293K |