4MLC
ABC Transporter Substrate-Binding Protein fromDesulfitobacterium hafniense
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.195, 81.796, 128.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.972 - 2.705 |
R-factor | 0.171 |
Rwork | 0.166 |
R-free | 0.21900 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 11736 | |
<I/σ(I)> | 15.1 | 4.35 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.2 M Calciam Acetate, 0.1 M sodium Caccodylate pH 6.5, 40 % (v/v) PEG300, VAPOR DIFFUSION, SITTING DROP, temperature 289K |