4ML4
Crystal structure of the complex of signaling glycoprotein from buffalo (SPB-40) with tetrahydropyran at 2.5 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-07-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.174, 67.088, 106.569 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.760 - 2.500 |
R-factor | 0.24741 |
Rwork | 0.247 |
R-free | 0.26329 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o9o |
RMSD bond length | 0.016 |
RMSD bond angle | 1.785 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.500 | 2.470 |
Number of reflections | 15750 | |
<I/σ(I)> | 19.1 | 3.1 |
Completeness [%] | 95.7 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 25mM Tris, 50mM NaCl, 20% ethanol, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |