4MKS
Crystal structure of enolase from Lactobacillus gasseri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9786 |
Spacegroup name | I 4 |
Unit cell lengths | 145.240, 145.240, 99.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.450 - 2.079 |
R-factor | 0.1914 |
Rwork | 0.189 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SWISSMODEL generated using 1w6t template |
RMSD bond length | 0.003 |
RMSD bond angle | 0.763 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (Autobuild) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 54.450 |
High resolution limit [Å] | 2.079 |
Number of reflections | 62294 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 297 | pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |