4MKF
Crystal structure of a stable adenylate kinase variant AKv3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.447, 75.008, 77.195 |
| Unit cell angles | 90.00, 100.89, 90.00 |
Refinement procedure
| Resolution | 26.990 - 1.700 |
| R-factor | 0.15904 |
| Rwork | 0.157 |
| R-free | 0.20271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p3j |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.253 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.515 | |
| Number of reflections | 42262 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295.15 | 36% (w/v) polyethylene glycol 1500, 50mM CaCl2, 50mM Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
