4MJX
Crystal structure of the double mutant (S112A, H303A) of B.anthracis mycrocine immunity protein (MccF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 |
| Unit cell lengths | 118.382, 118.382, 56.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.730 - 1.400 |
| R-factor | 0.11208 |
| Rwork | 0.110 |
| R-free | 0.14826 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gjz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.956 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.074 | |
| Number of reflections | 152732 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 289 | 1.4 M Sodium phosphate monobasic monohydrate/Potassium phosphate dibasic, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
