4MJN
Structure of the c ring of the CF1FO ATP synthases.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.420, 99.295, 123.510 |
| Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
| Resolution | 19.760 - 6.000 |
| R-factor | 0.35885 |
| Rwork | 0.358 |
| R-free | 0.37784 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w5j |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.105 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 6.320 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmerge | 0.049 | 0.106 |
| Number of reflections | 4091 | |
| <I/σ(I)> | 9.6 | 5.3 |
| Completeness [%] | 96.5 | 96.76 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.3 | 291 | 33%(v/v) PEG550 MME, 0.05M Sodium acetate, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
