4MJK

Crystal structure of a CRISPR protein from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	42.251, 87.524, 140.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.474 - 1.953
R-factor	0.1959
Rwork	0.195
R-free	0.21650
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.108
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.474
High resolution limit [Å]	1.953
Number of reflections	34700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	14% ethanol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K