4MJF

Crystal structure of a DUF4348 family protein (BVU_2238) from Bacteroides vulgatus ATCC 8482 at 1.99 A resolution

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-02
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 1 2 1
Unit cell lengths	64.719, 71.913, 65.504
Unit cell angles	90.00, 92.31, 90.00

Refinement procedure

Resolution	48.085 - 1.990
R-factor	0.1926
Rwork	0.191
R-free	0.21960
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.085		2.060
High resolution limit [Å]	1.990	4.270	1.990
Rmerge	0.037	0.030	0.562
Rmeas	0.044	0.036	0.669
Total number of observations	67280
Number of reflections	20233	2059	1999
<I/σ(I)>	15.21	34.7	2.1
Completeness [%]	97.6	95.9	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	277	0.200M lithium sulfate, 2.00M ammonium sulfate, 0.200M sodium thiocyanate, 0.1M CAPS pH 10.5, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K