4MJ7
Crystal structure of the PIN domain of Saccharomyces cerevisiae Utp23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97637 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 91.427, 91.427, 92.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.929 - 2.510 |
| R-factor | 0.2304 |
| Rwork | 0.228 |
| R-free | 0.28810 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.258 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.103 | 0.361 |
| Number of reflections | 15536 | |
| <I/σ(I)> | 30.5 | 6.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 8.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES, 2%(v/v) tacsimate, 20%(w/v) polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
