4MIV
Crystal Structure of Sulfamidase, Crystal Form L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99989 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 102.930, 211.450, 108.350 |
Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
Resolution | 48.850 - 2.400 |
R-factor | 0.2173 |
Rwork | 0.216 |
R-free | 0.24470 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.683 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.900 | 2.460 | |
High resolution limit [Å] | 2.400 | 10.730 | 2.400 |
Rmerge | 0.064 | 0.030 | 0.561 |
Number of reflections | 333757 | 3522 | 24389 |
<I/σ(I)> | 7.78 | 22.62 | 1.32 |
Completeness [%] | 95.6 | 89.7 | 94.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | 13% (w/v) polyethylene glycol (PEG) 8000, 200 mM MgCl2 and 100 mM Bis-Tris buffer, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |