4MIU
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-01-25 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.204, 60.554, 38.201 |
Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
Resolution | 38.140 - 1.670 |
R-factor | 0.1866 |
Rwork | 0.184 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.019 |
RMSD bond angle | 1.907 |
Data reduction software | APEX |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.770 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.015 | 0.184 |
Number of reflections | 16555 | |
<I/σ(I)> | 46.5 | 5.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.7 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |