4MIH
Pyranose 2-oxidase from Phanerochaete chrysosporium, recombinant H158A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.04094 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.100, 167.099, 168.472 |
Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
Resolution | 48.558 - 2.400 |
R-factor | 0.2058 |
Rwork | 0.205 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.165 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.560 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 233009 | |
<I/σ(I)> | 11.9 | 1.7 |
Completeness [%] | 98.4 | 97.4 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M HEPES, 0.2 M NH4Cl, 15% (w/v) PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |