4MHC
Crystal Structure of a Nucleoporin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0750 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.110, 76.940, 82.630 |
Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
Resolution | 45.100 - 2.400 |
R-factor | 0.1908 |
Rwork | 0.189 |
R-free | 0.23080 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (1.8.2_1309) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.105 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.068 | 0.427 |
Number of reflections | 28352 | |
<I/σ(I)> | 20 | 3.4 |
Completeness [%] | 96.4 | 74.2 |
Redundancy | 9.4 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 5% PEG 4,000, 0.1 M MES, pH 6.0, 0.05 M Magnesium chloride, 3% (v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |