4MHC
Crystal Structure of a Nucleoporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.110, 76.940, 82.630 |
| Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
| Resolution | 45.100 - 2.400 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.23080 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.849 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.8.2_1309) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.105 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.068 | 0.427 |
| Number of reflections | 28352 | |
| <I/σ(I)> | 20 | 3.4 |
| Completeness [%] | 96.4 | 74.2 |
| Redundancy | 9.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 5% PEG 4,000, 0.1 M MES, pH 6.0, 0.05 M Magnesium chloride, 3% (v/v) 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
