4MH4
Crystal Structure of OsmC-like protein from Burkholderia cenocepacia J2315
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 43 |
| Unit cell lengths | 87.940, 87.940, 44.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 1.900 |
| R-factor | 0.167 |
| Rwork | 0.166 |
| R-free | 0.19610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zb8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.493 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 | |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.037 | 0.018 | 0.419 |
| Number of reflections | 27321 | 319 | 2016 |
| <I/σ(I)> | 30.53 | 76.65 | 4.32 |
| Completeness [%] | 99.7 | 92.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | JCSG+(c1): 20%PEG-8000, 100mM Sodium Phosphate Dibasic/ Citric Acid 200mM Sodium Chloride, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
