4MH2
Crystal structure of Bovine Mitochondrial Peroxiredoxin III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.571, 260.825, 81.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.470 - 2.200 |
| R-factor | 0.18235 |
| Rwork | 0.180 |
| R-free | 0.21868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zye |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.501 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 258.200 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.089 | 0.738 |
| Number of reflections | 149669 | |
| <I/σ(I)> | 11.4 | 1.6 |
| Completeness [%] | 98.6 | 96.3 |
| Redundancy | 6.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 34% 2-Methyl-1,3-Propanediol, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
