4MGU

Crystal structure of Acheta domesticus Densovirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-28
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1
Spacegroup name	P 42 21 2
Unit cell lengths	415.469, 415.469, 281.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.500
R-factor	0.29
Rwork	0.290
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	GLRF
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.500
Number of reflections	306064
Completeness [%]	45.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	20% PEG 200, 200mM MgCl2, 100mM Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K