4MGR

The crystal structure of Bacillus subtilis GabR, an autorepressor and PLP- and GABA-dependent transcriptional activator of gabT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	99.930, 101.360, 213.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 2.550
R-factor	0.1967
Rwork	0.194
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.125
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.300
High resolution limit [Å]	2.430
Rmerge	0.118
Number of reflections	80839
<I/σ(I)>	10.6
Completeness [%]	97.6
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	33% PEG400, 200mM calcium acetate and 200mM imidazole, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K