4MG3
Crystal Structural Analysis of 2A Protease from Coxsackievirus A16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-17 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 101.271, 101.271, 193.914 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.470 - 1.798 |
R-factor | 0.1749 |
Rwork | 0.173 |
R-free | 0.20280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.268 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 | 1.860 |
High resolution limit [Å] | 1.798 | 2.270 | 1.800 |
Number of reflections | 35773 | ||
Completeness [%] | 99.8 | 98.3 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.1M MgCl2, 0.1M HEPES-Na (pH 7.5), 18% (w/v) PEG 400, hanging drop, temperature 289K, VAPOR DIFFUSION, HANGING DROP |