4MFV

Crystal structure of human CTNNBL1(residues 33~563)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-25
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97985
Spacegroup name	P 31
Unit cell lengths	90.037, 90.037, 175.746
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.840 - 2.920
R-factor	0.2364
Rwork	0.233
R-free	0.30530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mfu
RMSD bond length	0.010
RMSD bond angle	1.481
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.880	50.000	3.020
High resolution limit [Å]	2.920	6.290	2.920
Rmerge	0.078	0.052	0.266
Number of reflections	32475
<I/σ(I)>	30.3	87.7	2.6
Completeness [%]	94.1	98.9	77.6
Redundancy	7.2	8.8	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	295	PEG8000, CAPs, NaCl, pH 9.5, vapor diffusion, hanging drop, temperature 295K