4MFU
Crystal structure of human CTNNBL1(residues 77~563)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-30 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97985 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.149, 90.149, 178.557 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.330 - 2.744 |
R-factor | 0.2507 |
Rwork | 0.247 |
R-free | 0.31870 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.426 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.380 | 50.000 | 2.840 |
High resolution limit [Å] | 2.740 | 5.900 | 2.740 |
Rmerge | 0.095 | 0.080 | 0.328 |
Number of reflections | 22673 | ||
<I/σ(I)> | 36.9 | 112.2 | 2.2 |
Completeness [%] | 95.9 | 98.4 | 71.5 |
Redundancy | 13.9 | 19.2 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | PEG8000, CAPs, NaCl, pH 9.5, vapor diffusion, hanging drop, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 295 | PEG8000, CAPs, NaCl, pH 9.5, vapor diffusion, hanging drop, temperature 295K |