4MFR
Crystal structure of Mycobacterium tuberculosis CarD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.248, 93.248, 87.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.182 - 2.500 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.24290 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.507 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.730 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.131 | 0.655 |
| Number of reflections | 27954 | |
| <I/σ(I)> | 19.4 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 1.5M Lithium Sulphate, 0.1M HEPES pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
