4MFL

The crystal structure of acyltransferase in complex with decanoyl-CoA and Tei pseudoaglycone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A
Synchrotron site	NSRRC
Beamline	BL15A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-19
Detector	RAYONIX MX300HE
Wavelength(s)	1.00000
Spacegroup name	P 65
Unit cell lengths	133.581, 133.581, 49.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.1758
Rwork	0.174
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mfj
RMSD bond length	0.010
RMSD bond angle	1.302
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (for MR)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	37841
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1mM MES, 0.2M ammonium sulphate, 30%(V/V) PEG5000 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K