4MF2
Structure of human DNA polymerase beta complexed with O6MG as the template base in a 1-nucleotide gapped DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.438, 79.265, 54.789 |
Unit cell angles | 90.00, 105.66, 90.00 |
Refinement procedure
Resolution | 19.816 - 2.400 |
R-factor | 0.2022 |
Rwork | 0.198 |
R-free | 0.27910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.620 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 16418 |
Completeness [%] | 95.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 14% 23% PEG3400, and 350 mM sodium acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |