4MEW
Structure of the core fragment of human PR70
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.110, 74.460, 62.330 |
| Unit cell angles | 90.00, 92.63, 90.00 |
Refinement procedure
| Resolution | 37.230 - 1.993 |
| R-factor | 0.1635 |
| Rwork | 0.162 |
| R-free | 0.19310 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.803 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX ((autosol)) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.230 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 45116 | |
| <I/σ(I)> | 13.8 | 2.42 |
| Completeness [%] | 99.1 | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 277 | 0.1 M Tris-HCl, 16% PEG 2000 monomethyl ether, 0.15 M trimethylamine N-oxide, pH 7.5, VAPOR DIFFUSION, temperature 277K |
