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4MER

Crystal structure of the novel protein and virulence factor sHIP (Q99XU0) from Streptococcus pyogenes

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsBESSY BEAMLINE 14.1
Synchrotron siteBESSY
Beamline14.1
Temperature [K]100
Detector technologyCCD
Collection date2012-03-08
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.91841
Spacegroup nameP 1 21 1
Unit cell lengths49.547, 111.222, 51.136
Unit cell angles90.00, 108.02, 90.00
Refinement procedure
Resolution48.630 - 2.410
R-factor0.23077
Rwork0.229
R-free0.26917
Structure solution methodSIRAS
RMSD bond length0.014
RMSD bond angle1.654
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwareautoSHARP
Refinement softwareREFMAC (5.7.0029)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.500
High resolution limit [Å]2.4102.410
Number of reflections20249
Completeness [%]98.0
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.529120% PEG3350, 0.2 M sodium malonate, 0.1 M Bis-Tris propane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

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