4MEM
Crystal Structure of the rat USP11 DUSP-UBL domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.973 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 37.285, 80.156, 149.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.622 - 2.340 |
| R-factor | 0.2228 |
| Rwork | 0.221 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mel |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.916 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 74.635 |
| High resolution limit [Å] | 2.292 |
| Number of reflections | 20914 |
| Completeness [%] | 99.7 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.77 | 292 | 0.15 M Malic Acid, 24% w/v PEG 3350, 10 mM Strontium Chloride, pH 6.77, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
