4ME8

Crystal structure of a signal peptidase I (EF3073) from Enterococcus faecalis V583 at 2.27 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97944,0.97894
Spacegroup name	P 41 21 2
Unit cell lengths	37.488, 37.488, 169.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.174 - 2.270
R-factor	0.2162
Rwork	0.212
R-free	0.25800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.229
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.174		2.350
High resolution limit [Å]	2.270	4.870	2.270
Rmerge	0.077	0.033	0.777
Number of reflections	6200	1094	1057
<I/σ(I)>	11.93	30.8	1.7
Completeness [%]	99.4	98.3	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	20.00% polyethylene glycol 3350, 0.200M ammonium fluoride, No Buffer pH 6.2, Additive: 0.001 M leupeptin, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 293K