4MDX

Crystal structure of Bacillus subtilis MazF in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	66.793, 69.885, 79.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.554 - 1.500
R-factor	0.1648
Rwork	0.164
R-free	0.17760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.088
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.082	0.047	0.531
Number of reflections	60026
<I/σ(I)>	7.1
Completeness [%]	99.9	99.7	100
Redundancy	4.7	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.2 M sodium iodide, 20% w/v PEG 3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K