4MDW
Crystal structure of a DUF1541 family protein (ydhK) from Bacillus subtilis subsp. subtilis str. 168 at 2.00 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-08-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97926,0.97885 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.692, 64.174, 80.499 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.692 - 2.000 |
R-factor | 0.1824 |
Rwork | 0.179 |
R-free | 0.25970 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.692 | 2.070 | |
High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
Rmerge | 0.090 | 0.030 | 0.692 |
Number of reflections | 9897 | 1793 | 1527 |
<I/σ(I)> | 7.1 | 21.1 | 1.4 |
Completeness [%] | 94.7 | 94.1 | 83.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 5.0% polyethylene glycol 3000, 40.0% polyethylene glycol 400, 0.1M MES pH 6.0, NANODROP', VAPOR DIFFUSION, SITTING DROP, temperature 277K |