4MDF
Structure of bacterial polynucleotide kinase Michaelis complex bound to GTP and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2013-07-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.025, 72.755, 119.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.465 - 1.727 |
R-factor | 0.1658 |
Rwork | 0.163 |
R-free | 0.19600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.126 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.000 | 1.760 |
High resolution limit [Å] | 1.727 | 1.727 |
Number of reflections | 49311 | |
Completeness [%] | 100.0 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 100 mM sodium citrate, pH 5.0, 2 mM guanosine triphosphate, 100mM magnesium chloride,18-24% PEG 6000,1.1 mM short DNA strand, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |