4MD2
Ground state of bacteriorhodopsin from Halobacterium salinarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.910, 60.910, 110.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.440 - 1.730 |
| R-factor | 0.142 |
| Rwork | 0.139 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.440 | 1.820 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.049 | 0.342 |
| Number of reflections | 22537 | |
| <I/σ(I)> | 12.7 | 2.7 |
| Completeness [%] | 93.9 | 95.3 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | in meso | 5.6 | 295 | Crystals were obtained in a lipidic cubic phase of monoolein, pH 5.6, in meso, temperature 295K |
