4MCM
Human SOD1 C57S Mutant, As-isolated
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-19 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.530, 163.883, 174.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.836 - 2.200 |
R-factor | 0.213 |
Rwork | 0.210 |
R-free | 0.26210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1azv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.154 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 108701 | |
<I/σ(I)> | 25.6 | 2.9 |
Completeness [%] | 99.3 | 95.3 |
Redundancy | 6.1 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M ammonium sulfate, 0.1 M bis-tris, 25% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |