4MCK

Crystal structure of Family GH19, Class IV chitinase from Zea mays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	37.152, 65.167, 72.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.915 - 1.500
R-factor	0.1802
Rwork	0.178
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hbd
RMSD bond length	0.007
RMSD bond angle	1.248
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.416
High resolution limit [Å]	1.500	1.500
Rmerge	0.044
Number of reflections	28893
<I/σ(I)>		3.3
Completeness [%]	96.7	63.2
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.5 M ammonium sulfate, 0.1 M Tris, 0.16 M potassium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K