4MBU
Crystal structure of N-acetyltransferase from Staphylococcus aureus Mu50
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 97.490, 78.860, 66.010 |
Unit cell angles | 90.00, 111.95, 90.00 |
Refinement procedure
Resolution | 33.150 - 2.150 |
R-factor | 0.1873 |
Rwork | 0.185 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.021 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CCP4 |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.520 |
High resolution limit [Å] | 2.150 |
Number of reflections | 23855 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 0.047 M cadmium sulfate hydrate, 0.094 M HEPES pH 7.5,0.94 M sodium acetate trihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 296K |