4MBU

Crystal structure of N-acetyltransferase from Staphylococcus aureus Mu50

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	97.490, 78.860, 66.010
Unit cell angles	90.00, 111.95, 90.00

Refinement procedure

Resolution	33.150 - 2.150
R-factor	0.1873
Rwork	0.185
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.021
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.520
High resolution limit [Å]	2.150
Number of reflections	23855
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	0.047 M cadmium sulfate hydrate, 0.094 M HEPES pH 7.5,0.94 M sodium acetate trihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 296K