4MBO

1.65 Angstrom Crystal Structure of Serine-rich Repeat Adhesion Glycoprotein (Srr1) from Streptococcus agalactiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97928
Spacegroup name	P 21 21 2
Unit cell lengths	73.356, 83.235, 57.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.930 - 1.650
R-factor	0.15537
Rwork	0.154
R-free	0.18721
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r17
RMSD bond length	0.012
RMSD bond angle	1.554
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0046)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.058	0.500
Number of reflections	42747
<I/σ(I)>	26.4	3.8
Completeness [%]	99.5	100
Redundancy	6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	Protein: 7.6mg/mL, 0.5M Sodium cloride, 0.01M Tris-HCl, pH 8.3. Screen: PACT (B11), 0.2M Calcium chloride, 0.1M Tris-HCl, pH 6.0, 20% (w/v) PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 295K