4MB8
Evolutionary history and metabolic insights of ancient mammalian uricases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-12-12 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 143.778, 143.778, 138.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.767 - 2.401 |
R-factor | 0.1768 |
Rwork | 0.174 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.240 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1458) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.090 | 0.085 | 0.476 |
Number of reflections | 63527 | ||
<I/σ(I)> | 14.7 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 5.2 | 5.2 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 25% MPD, 40 mM MgCl2, and 50 mM sodium cacodylate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |