4MAY
Crystal structure of an immune complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.514, 123.176, 134.088 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.259 - 2.200 |
| R-factor | 0.1984 |
| Rwork | 0.196 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.191 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.090 | 0.035 | 0.857 |
| Number of reflections | 61418 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 99.6 | 97.4 | 99.7 |
| Redundancy | 4.5 | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 291 | 50mM Citrate buffer, 10% PEG8000,0.12M Ammonium Sulfate, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
