4MAU
Crystal structure of anti-ST2L antibody C2244
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-11-12 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.680, 113.360, 128.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.18462 |
| Rwork | 0.184 |
| R-free | 0.21746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1IBG AND 1F8T |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.059 | 0.246 |
| Number of reflections | 49460 | |
| <I/σ(I)> | 37.9 | 6.4 |
| Completeness [%] | 99.3 | 94.4 |
| Redundancy | 13.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1 M sodium acetate, pH 4.5, 2.3 M ammonium sulfate, 5% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
