4MAT
E.COLI METHIONINE AMINOPEPTIDASE HIS79ALA MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-10 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.648, 68.298, 55.449 |
Unit cell angles | 90.00, 105.98, 90.00 |
Refinement procedure
Resolution | 28.800 - 2.000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2mat |
RMSD bond length | 0.012 |
RMSD bond angle | 17.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT (5F PRERELEASE) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.800 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.047 * | 0.210 * |
Total number of observations | 48052 * | |
Number of reflections | 16062 | |
<I/σ(I)> | 22.4 | 4.4 |
Completeness [%] | 85.2 | 70.8 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | macroseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.7 (mg/ml) | |
2 | 1 | drop | dithiothreitol | 20 (mM) | |
3 | 1 | reservoir | PEG3400 | 22-27 (%) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | |
5 | 1 | reservoir | 200 (mM) |