4MAM
The crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit of Francisella tularensis subsp. tularensis SCHU S4 in complex with an ADP analog, AMP-CP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.241, 85.393, 127.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.493 - 1.474 |
| R-factor | 0.1606 |
| Rwork | 0.160 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m9u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.150 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.500 | 1.510 |
| High resolution limit [Å] | 1.474 | 1.480 |
| Rmerge | 0.068 | 0.595 |
| Number of reflections | 133621 | |
| <I/σ(I)> | 39.8 | 2.6 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 5.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M Sodium Acetate, 0.1M HEPES, 22% (w/v) PEG 4000, 15mM AM-CP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
